Construct: shRNA TRCN0000348578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020606.5-1821s21c1
DNA Barcode:
GCGAAGGTTTGCTATCCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parva (57342)
Gene Description:
parvin, alpha
Transcript:
RefSeq NM_020606.5 (CURRENT)
Match location:
Position 1821 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAGGTTTGCTATCCATTA
Hairpin Sequence:
5'-CCGG-GCGAAGGTTTGCTATCCATTA-CTCGAG-TAATGGATAGCAAACCTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAGGTTTGCTATCCATTACTCGAGTAATGGATAGCAAACCTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAGGTTTGCTATCCATTACTCGAGTAATGGATAGCAAACCTTCGC-3'

Other clones with same target sequence:

(none)