Construct: shRNA TRCN0000348586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011520.3-465s21c1
DNA Barcode:
ATGCTACCCACAACCGTTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sdc3 (20970)
Gene Description:
syndecan 3
Transcript:
RefSeq NM_011520.3 (CURRENT)
Match location:
Position 465 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCTACCCACAACCGTTATC
Hairpin Sequence:
5'-CCGG-ATGCTACCCACAACCGTTATC-CTCGAG-GATAACGGTTGTGGGTAGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCTACCCACAACCGTTATCCTCGAGGATAACGGTTGTGGGTAGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCTACCCACAACCGTTATCCTCGAGGATAACGGTTGTGGGTAGCAT-3'

Other clones with same target sequence:

(none)