Construct: shRNA TRCN0000348729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009231.2-1404s21c1
DNA Barcode:
TTTGACCCGTATGAGTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sos1 (20662)
Gene Description:
son of sevenless homolog 1 (Drosophila)
Transcript:
RefSeq NM_009231.2 (CURRENT)
Match location:
Position 1404 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGACCCGTATGAGTCATAT
Hairpin Sequence:
5'-CCGG-TTTGACCCGTATGAGTCATAT-CTCGAG-ATATGACTCATACGGGTCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGACCCGTATGAGTCATATCTCGAGATATGACTCATACGGGTCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGACCCGTATGAGTCATATCTCGAGATATGACTCATACGGGTCAAA-3'

Other clones with same target sequence:

(none)