Construct: shRNA TRCN0000348945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019771.2-766s21c1
DNA Barcode:
AGCTAGGTGGCTCCTTAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dstn (56431)
Gene Description:
destrin
Transcript:
RefSeq NM_019771.2 (CURRENT)
Match location:
Position 766 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTAGGTGGCTCCTTAATTG
Hairpin Sequence:
5'-CCGG-AGCTAGGTGGCTCCTTAATTG-CTCGAG-CAATTAAGGAGCCACCTAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTAGGTGGCTCCTTAATTGCTCGAGCAATTAAGGAGCCACCTAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTAGGTGGCTCCTTAATTGCTCGAGCAATTAAGGAGCCACCTAGCT-3'

Other clones with same target sequence:

(none)