Construct: shRNA TRCN0000349003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008900.2-210s21c1
DNA Barcode:
GATGGTCCAGAGCGACTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pou3f3 (18993)
Gene Description:
POU domain, class 3, transcription factor 3
Transcript:
RefSeq NM_008900.2 (CURRENT)
Match location:
Position 210 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTCCAGAGCGACTTCAT
Hairpin Sequence:
5'-CCGG-GATGGTCCAGAGCGACTTCAT-CTCGAG-ATGAAGTCGCTCTGGACCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTCCAGAGCGACTTCATCTCGAGATGAAGTCGCTCTGGACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTCCAGAGCGACTTCATCTCGAGATGAAGTCGCTCTGGACCATC-3'

Other clones with same target sequence:

  1. TRCN0000005753