Construct: shRNA TRCN0000349530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011875.4-539s21c1
DNA Barcode:
GTTTCTATGATCTCTCCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmd13 (23997)
Gene Description:
proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Transcript:
RefSeq NM_011875.4 (CURRENT)
Match location:
Position 539 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCTATGATCTCTCCAGTA
Hairpin Sequence:
5'-CCGG-GTTTCTATGATCTCTCCAGTA-CTCGAG-TACTGGAGAGATCATAGAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCTATGATCTCTCCAGTACTCGAGTACTGGAGAGATCATAGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCTATGATCTCTCCAGTACTCGAGTACTGGAGAGATCATAGAAAC-3'

Other clones with same target sequence:

  1. TRCN0000066022