Construct: shRNA TRCN0000349760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011127.2-2005s21c1
DNA Barcode:
CTATCCATGCTTAGCTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prrx1 (18933)
Gene Description:
paired related homeobox 1
Transcript:
RefSeq NM_011127.2 (CURRENT)
Match location:
Position 2005 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATCCATGCTTAGCTAATTA
Hairpin Sequence:
5'-CCGG-CTATCCATGCTTAGCTAATTA-CTCGAG-TAATTAGCTAAGCATGGATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATCCATGCTTAGCTAATTACTCGAGTAATTAGCTAAGCATGGATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATCCATGCTTAGCTAATTACTCGAGTAATTAGCTAAGCATGGATAG-3'

Other clones with same target sequence:

(none)