Construct: shRNA TRCN0000349817
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011631.1-1575s21c1
- DNA Barcode:
- GTGATTGAAGACCACTCAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hsp90b1 (22027)
- Gene Description:
- heat shock protein 90, beta (Grp94), member 1
- Transcript:
- RefSeq NM_011631.1 (CURRENT)
- Match location:
- Position 1575 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000349817)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTGATTGAAGACCACTCAAAT
- Hairpin Sequence:
-
5'-CCGG-GTGATTGAAGACCACTCAAAT-CTCGAG-ATTTGAGTGGTCTTCAATCAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTGATTGAAGACCACTCAAATCTCGAGATTTGAGTGGTCTTCAATCACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTGATTGAAGACCACTCAAATCTCGAGATTTGAGTGGTCTTCAATCAC-3'
Other clones with same target sequence:
(none)
