Construct: shRNA TRCN0000349938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010162.2-3154s21c1
DNA Barcode:
AGCCAGACTGTGCCAAGTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ext1 (14042)
Gene Description:
exostoses (multiple) 1
Transcript:
RefSeq NM_010162.2 (CURRENT)
Match location:
Position 3154 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000349938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCAGACTGTGCCAAGTATC
Hairpin Sequence:
5'-CCGG-AGCCAGACTGTGCCAAGTATC-CTCGAG-GATACTTGGCACAGTCTGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCAGACTGTGCCAAGTATCCTCGAGGATACTTGGCACAGTCTGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCAGACTGTGCCAAGTATCCTCGAGGATACTTGGCACAGTCTGGCT-3'

Other clones with same target sequence:

(none)