Transcript: Mouse NM_010162.2

Mus musculus exostoses (multiple) 1 (Ext1), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Ext1 (14042)
Length:
3478
CDS:
821..3061

Additional Resources:

NCBI RefSeq record:
NM_010162.2
NBCI Gene record:
Ext1 (14042)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010162.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000093789 CCCTTACTACTATGCTAATTT pLKO.1 2215 CDS 100% 15.000 21.000 N Ext1 n/a
2 TRCN0000317457 CCCTTACTACTATGCTAATTT pLKO_005 2215 CDS 100% 15.000 21.000 N Ext1 n/a
3 TRCN0000313800 GTTCTTGTGGGAGGCTTATTT pLKO_005 2047 CDS 100% 15.000 21.000 N Ext1 n/a
4 TRCN0000313799 TGTTCGTCCTACCGCAGTATT pLKO_005 2175 CDS 100% 13.200 18.480 N Ext1 n/a
5 TRCN0000093791 GCAGATCATAGTTCTGTGGAA pLKO.1 2353 CDS 100% 2.640 3.696 N Ext1 n/a
6 TRCN0000093790 CCTTCTACAATCAGGTCTATT pLKO.1 1988 CDS 100% 13.200 10.560 N Ext1 n/a
7 TRCN0000349517 CCTTCTACAATCAGGTCTATT pLKO_005 1988 CDS 100% 13.200 10.560 N Ext1 n/a
8 TRCN0000349938 AGCCAGACTGTGCCAAGTATC pLKO_005 3154 3UTR 100% 10.800 7.560 N Ext1 n/a
9 TRCN0000093792 CCAGAGAGGATTGTGGGATAT pLKO.1 2576 CDS 100% 10.800 7.560 N Ext1 n/a
10 TRCN0000039994 CCTGCTTCGATTTCACCCTTT pLKO.1 1125 CDS 100% 4.050 2.835 N EXT1 n/a
11 TRCN0000039996 GCACTTAGACAGCAGACACAA pLKO.1 2027 CDS 100% 4.950 3.465 N EXT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010162.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00523 pDONR223 100% 95.8% 99.3% None (many diffs) n/a
2 ccsbBroad304_00523 pLX_304 0% 95.8% 99.3% V5 (many diffs) n/a
3 TRCN0000472228 GTTCGTTTTGTTCTCAACTAATGG pLX_317 18.6% 95.8% 99.3% V5 (many diffs) n/a
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