Construct: shRNA TRCN0000350170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026376.3-4558s21c1
DNA Barcode:
ACGGCAAGCTGGAGTACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxnd1 (67784)
Gene Description:
plexin D1
Transcript:
RefSeq NM_026376.3 (CURRENT)
Match location:
Position 4558 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGCAAGCTGGAGTACTATA
Hairpin Sequence:
5'-CCGG-ACGGCAAGCTGGAGTACTATA-CTCGAG-TATAGTACTCCAGCTTGCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGCAAGCTGGAGTACTATACTCGAGTATAGTACTCCAGCTTGCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGCAAGCTGGAGTACTATACTCGAGTATAGTACTCCAGCTTGCCGT-3'

Other clones with same target sequence:

(none)