Construct: shRNA TRCN0000350789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145659.1-2406s21c1
DNA Barcode:
AGCAAGACACCTGACAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTAGE9 (643854)
Gene Description:
CTAGE family member 9
Transcript:
RefSeq NM_001145659.1 (CURRENT)
Match location:
Position 2406 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGACACCTGACAATATT
Hairpin Sequence:
5'-CCGG-AGCAAGACACCTGACAATATT-CTCGAG-AATATTGTCAGGTGTCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGACACCTGACAATATTCTCGAGAATATTGTCAGGTGTCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGACACCTGACAATATTCTCGAGAATATTGTCAGGTGTCTTGCT-3'

Other clones with same target sequence:

(none)