Construct: shRNA TRCN0000351064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198168.3-1328s21c1
DNA Barcode:
ACGAGCTGGAACACTTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r5b (225849)
Gene Description:
protein phosphatase 2, regulatory subunit B', beta
Transcript:
RefSeq NM_198168.3 (CURRENT)
Match location:
Position 1328 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAGCTGGAACACTTCAATG
Hairpin Sequence:
5'-CCGG-ACGAGCTGGAACACTTCAATG-CTCGAG-CATTGAAGTGTTCCAGCTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAGCTGGAACACTTCAATGCTCGAGCATTGAAGTGTTCCAGCTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAGCTGGAACACTTCAATGCTCGAGCATTGAAGTGTTCCAGCTCGT-3'

Other clones with same target sequence:

(none)