Construct: shRNA TRCN0000351791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146015.2-1565s21c1
DNA Barcode:
GCTCTGTGTTAAGATTGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efemp1 (216616)
Gene Description:
epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Transcript:
RefSeq NM_146015.2 (CURRENT)
Match location:
Position 1565 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGTGTTAAGATTGACAA
Hairpin Sequence:
5'-CCGG-GCTCTGTGTTAAGATTGACAA-CTCGAG-TTGTCAATCTTAACACAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGTGTTAAGATTGACAACTCGAGTTGTCAATCTTAACACAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGTGTTAAGATTGACAACTCGAGTTGTCAATCTTAACACAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000109681