Construct: shRNA TRCN0000351948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173733.3-1732s21c1
DNA Barcode:
GCACCATTTGTACCCAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Suox (211389)
Gene Description:
sulfite oxidase
Transcript:
RefSeq NM_173733.3 (CURRENT)
Match location:
Position 1732 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCATTTGTACCCAAAGAA
Hairpin Sequence:
5'-CCGG-GCACCATTTGTACCCAAAGAA-CTCGAG-TTCTTTGGGTACAAATGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCATTTGTACCCAAAGAACTCGAGTTCTTTGGGTACAAATGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCATTTGTACCCAAAGAACTCGAGTTCTTTGGGTACAAATGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000076663