Construct: shRNA TRCN0000352097

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145467.2-1476s21c1
DNA Barcode:
GTTCTGGAGAAGAGTGGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itgbl1 (223272)
Gene Description:
integrin, beta-like 1
Transcript:
RefSeq NM_145467.2 (CURRENT)
Match location:
Position 1476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352097)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTGGAGAAGAGTGGTATA
Hairpin Sequence:
5'-CCGG-GTTCTGGAGAAGAGTGGTATA-CTCGAG-TATACCACTCTTCTCCAGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTGGAGAAGAGTGGTATACTCGAGTATACCACTCTTCTCCAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTGGAGAAGAGTGGTATACTCGAGTATACCACTCTTCTCCAGAAC-3'

Other clones with same target sequence:

  1. TRCN0000094078