Construct: shRNA TRCN0000353151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017829.5-1158s21c1
DNA Barcode:
ACGTGGTGAATGACGTGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDHD5 (27440)
Gene Description:
haloacid dehalogenase like hydrolase domain containing 5
Transcript:
RefSeq NM_017829.5 (CURRENT)
Match location:
Position 1158 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGGTGAATGACGTGAATG
Hairpin Sequence:
5'-CCGG-ACGTGGTGAATGACGTGAATG-CTCGAG-CATTCACGTCATTCACCACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGGTGAATGACGTGAATGCTCGAGCATTCACGTCATTCACCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGGTGAATGACGTGAATGCTCGAGCATTCACGTCATTCACCACGT-3'

Other clones with same target sequence:

(none)