Construct: shRNA TRCN0000353181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007638.4-2294s21c1
DNA Barcode:
GATGAGAAGATGGTTGCTGGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct7 (12468)
Gene Description:
chaperonin containing Tcp1, subunit 7 (eta)
Transcript:
RefSeq NM_007638.4 (CURRENT)
Match location:
Position 2294 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGAAGATGGTTGCTGGT
Hairpin Sequence:
5'-CCGG-GATGAGAAGATGGTTGCTGGT-CTCGAG-ACCAGCAACCATCTTCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGAAGATGGTTGCTGGTCTCGAGACCAGCAACCATCTTCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGAAGATGGTTGCTGGTCTCGAGACCAGCAACCATCTTCTCATC-3'

Other clones with same target sequence:

  1. TRCN0000120457