Construct: shRNA TRCN0000353521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009075.2-809s21c1
DNA Barcode:
GAGTGAAGTGAACACAGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpia (19895)
Gene Description:
ribose 5-phosphate isomerase A
Transcript:
RefSeq NM_009075.2 (CURRENT)
Match location:
Position 809 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGAAGTGAACACAGCTAT
Hairpin Sequence:
5'-CCGG-GAGTGAAGTGAACACAGCTAT-CTCGAG-ATAGCTGTGTTCACTTCACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGAAGTGAACACAGCTATCTCGAGATAGCTGTGTTCACTTCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGAAGTGAACACAGCTATCTCGAGATAGCTGTGTTCACTTCACTC-3'

Other clones with same target sequence:

  1. TRCN0000178416