Transcript: Mouse NM_009075.2

Mus musculus ribose 5-phosphate isomerase A (Rpia), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Rpia (19895)
Length:
1789
CDS:
27..938

Additional Resources:

NCBI RefSeq record:
NM_009075.2
NBCI Gene record:
Rpia (19895)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353459 GGTGACGGATAACGGGAATTT pLKO_005 752 CDS 100% 13.200 18.480 N Rpia n/a
2 TRCN0000197395 CTTATCTTGCAGTATGGCTTA pLKO.1 417 CDS 100% 4.050 5.670 N Rpia n/a
3 TRCN0000181790 GTCTACTTTGGGATGCAGGAT pLKO.1 885 CDS 100% 2.640 3.696 N Rpia n/a
4 TRCN0000328576 GTCTACTTTGGGATGCAGGAT pLKO_005 885 CDS 100% 2.640 3.696 N Rpia n/a
5 TRCN0000197623 CCTTATGGGAAACCTTGTTTA pLKO.1 1362 3UTR 100% 13.200 10.560 N Rpia n/a
6 TRCN0000216494 CAAGTCGTTTCATTGTGATTG pLKO.1 571 CDS 100% 10.800 8.640 N Rpia n/a
7 TRCN0000217209 CCACTCCCAAATCTTAGTTAC pLKO.1 1423 3UTR 100% 10.800 7.560 N Rpia n/a
8 TRCN0000181286 CTGGATCAACACCCAGAGATT pLKO.1 450 CDS 100% 4.950 3.465 N Rpia n/a
9 TRCN0000049410 GAATTGGAAGTGGTTCTACAA pLKO.1 307 CDS 100% 4.950 3.465 N RPIA n/a
10 TRCN0000363585 GAATTGGAAGTGGTTCTACAA pLKO_005 307 CDS 100% 4.950 3.465 N RPIA n/a
11 TRCN0000178416 GAGTGAAGTGAACACAGCTAT pLKO.1 809 CDS 100% 4.950 2.970 N Rpia n/a
12 TRCN0000353521 GAGTGAAGTGAACACAGCTAT pLKO_005 809 CDS 100% 4.950 2.970 N Rpia n/a
13 TRCN0000181768 GCAGCGAATAGCTGAAAGAGT pLKO.1 341 CDS 100% 3.000 1.800 N Rpia n/a
14 TRCN0000353458 GCAGCGAATAGCTGAAAGAGT pLKO_005 341 CDS 100% 3.000 1.800 N Rpia n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11653 pDONR223 100% 70.7% 73.3% None (many diffs) n/a
2 ccsbBroad304_11653 pLX_304 0% 70.7% 73.3% V5 (many diffs) n/a
3 TRCN0000465621 AACAAAACTCGTAACTTCCCCATC pLX_317 52.8% 70.7% 73.3% V5 (many diffs) n/a
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