Construct: shRNA TRCN0000354105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029519.3-687s21c1
DNA Barcode:
CCCTTTATGGAGACTTCTGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rap2a (76108)
Gene Description:
RAS related protein 2a
Transcript:
RefSeq NM_029519.3 (CURRENT)
Match location:
Position 687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000354105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTATGGAGACTTCTGCT
Hairpin Sequence:
5'-CCGG-CCCTTTATGGAGACTTCTGCT-CTCGAG-AGCAGAAGTCTCCATAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTATGGAGACTTCTGCTCTCGAGAGCAGAAGTCTCCATAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTATGGAGACTTCTGCTCTCGAGAGCAGAAGTCTCCATAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000102799