Construct: shRNA TRCN0000356432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013938.1-259s21c1
DNA Barcode:
CTCTTCACCCATCGTTCCATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10H3 (26532)
Gene Description:
olfactory receptor family 10 subfamily H member 3
Transcript:
RefSeq NM_013938.1 (CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTCACCCATCGTTCCATC
Hairpin Sequence:
5'-CCGG-CTCTTCACCCATCGTTCCATC-CTCGAG-GATGGAACGATGGGTGAAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTCACCCATCGTTCCATCCTCGAGGATGGAACGATGGGTGAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTCACCCATCGTTCCATCCTCGAGGATGGAACGATGGGTGAAGAG-3'

Other clones with same target sequence:

(none)