Construct: shRNA TRCN0000359845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004704.1-155s21c1
DNA Barcode:
GTCTGAGGTCACCTATGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4C6 (219432)
Gene Description:
olfactory receptor family 4 subfamily C member 6
Transcript:
RefSeq NM_001004704.1 (CURRENT)
Match location:
Position 155 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000359845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGAGGTCACCTATGTATT
Hairpin Sequence:
5'-CCGG-GTCTGAGGTCACCTATGTATT-CTCGAG-AATACATAGGTGACCTCAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGAGGTCACCTATGTATTCTCGAGAATACATAGGTGACCTCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGAGGTCACCTATGTATTCTCGAGAATACATAGGTGACCTCAGAC-3'

Other clones with same target sequence:

(none)