Construct: shRNA TRCN0000360120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173492.1-679s21c1
DNA Barcode:
GAGCACCTGTGGAAGACACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5KL1 (138429)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase like 1
Transcript:
RefSeq NM_173492.1 (CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCACCTGTGGAAGACACTG
Hairpin Sequence:
5'-CCGG-GAGCACCTGTGGAAGACACTG-CTCGAG-CAGTGTCTTCCACAGGTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCACCTGTGGAAGACACTGCTCGAGCAGTGTCTTCCACAGGTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCACCTGTGGAAGACACTGCTCGAGCAGTGTCTTCCACAGGTGCTC-3'

Other clones with same target sequence:

(none)