Transcript: Human NM_173492.1

Homo sapiens phosphatidylinositol-4-phosphate 5-kinase like 1 (PIP5KL1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-03-20
Taxon:
Homo sapiens (human)
Gene:
PIP5KL1 (138429)
Length:
1131
CDS:
211..786

Additional Resources:

NCBI RefSeq record:
NM_173492.1
NBCI Gene record:
PIP5KL1 (138429)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146428 CAGGGCAAGACCATCAACCT pXPR_003 GGG 149 26% 3 0.8055 PIP5KL1 PIP5KL1 75823
2 BRDN0001145139 CAGGGGCGGGATCCACCCAG pXPR_003 CGG 74 13% 3 0.6798 PIP5KL1 PIP5KL1 75822
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_173492.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199024 CCCTAACTAATACCGTAACCG pLKO.1 905 3UTR 100% 2.640 3.696 N PIP5KL1 n/a
2 TRCN0000360119 TTCCAACGTCTCCACGAGGAT pLKO_005 457 CDS 100% 2.640 3.696 N PIP5KL1 n/a
3 TRCN0000360122 TAATACCGTAACCGCGCAGTC pLKO_005 912 3UTR 100% 2.250 3.150 N PIP5KL1 n/a
4 TRCN0000052538 CCCTACTTTAGCTCATTTAAT pLKO.1 976 3UTR 100% 15.000 12.000 N PIP5KL1 n/a
5 TRCN0000234458 CCCTACTTTAGCTCATTTAAT pLKO_005 976 3UTR 100% 15.000 12.000 N PIP5KL1 n/a
6 TRCN0000234454 CCGAGAGGTATGACATCAAAG pLKO_005 245 CDS 100% 10.800 8.640 N PIP5KL1 n/a
7 TRCN0000234455 TGCTGAAGGACCTCAACTTTC pLKO_005 323 CDS 100% 10.800 8.640 N PIP5KL1 n/a
8 TRCN0000362235 TGCTGAAGGACCTCAACTTTC pLKO_005 323 CDS 100% 10.800 8.640 N Pip5kl1 n/a
9 TRCN0000052539 TCCGAGAGGTATGACATCAAA pLKO.1 244 CDS 100% 5.625 4.500 N PIP5KL1 n/a
10 TRCN0000234456 ATGGAACTGGATACCACCTTC pLKO_005 394 CDS 100% 4.050 3.240 N PIP5KL1 n/a
11 TRCN0000360120 GAGCACCTGTGGAAGACACTG pLKO_005 679 CDS 100% 1.350 1.080 N PIP5KL1 n/a
12 TRCN0000052540 GTGCTGAAGGACCTCAACTTT pLKO.1 322 CDS 100% 5.625 3.938 N PIP5KL1 n/a
13 TRCN0000360121 ACTCTCCGGATCTGGACGATG pLKO_005 802 3UTR 100% 1.350 0.945 N PIP5KL1 n/a
14 TRCN0000052542 CTCCGCCAGATGGAACTGGAT pLKO.1 385 CDS 100% 0.880 0.616 N PIP5KL1 n/a
15 TRCN0000052541 CCTGTGGAAGACACTGCGCTA pLKO.1 684 CDS 100% 0.720 0.504 N PIP5KL1 n/a
16 TRCN0000199721 GCAGTCCCGTTTGACGGTGGT pLKO.1 927 3UTR 100% 0.000 0.000 N PIP5KL1 n/a
17 TRCN0000234457 TGGATTACAGCCTCCTGATAG pLKO_005 434 CDS 100% 10.800 6.480 N PIP5KL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173492.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04920 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04920 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466485 TAAAACAGTGGTCAGGCCGCTACT pLX_317 36.5% 100% 100% V5 n/a
4 ccsbBroadEn_15249 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_15249 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000480229 CAATGCAGAGAGTCTTCGACTCCA pLX_317 45.3% 100% 100% V5 n/a
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