Construct: shRNA TRCN0000360163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206997.1-726s21c1
DNA Barcode:
ACCATTCTGTCAGCCTATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR152 (390212)
Gene Description:
G protein-coupled receptor 152
Transcript:
RefSeq NM_206997.1 (CURRENT)
Match location:
Position 726 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATTCTGTCAGCCTATGTG
Hairpin Sequence:
5'-CCGG-ACCATTCTGTCAGCCTATGTG-CTCGAG-CACATAGGCTGACAGAATGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATTCTGTCAGCCTATGTGCTCGAGCACATAGGCTGACAGAATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATTCTGTCAGCCTATGTGCTCGAGCACATAGGCTGACAGAATGGT-3'

Other clones with same target sequence:

(none)