Construct: shRNA TRCN0000360870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037294.1-3884s21c1
DNA Barcode:
GCGGCAAAGTGAAGATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alpk2 (225638)
Gene Description:
alpha-kinase 2
Transcript:
RefSeq NM_001037294.1 (CURRENT)
Match location:
Position 3884 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCAAAGTGAAGATCATAA
Hairpin Sequence:
5'-CCGG-GCGGCAAAGTGAAGATCATAA-CTCGAG-TTATGATCTTCACTTTGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCAAAGTGAAGATCATAACTCGAGTTATGATCTTCACTTTGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCAAAGTGAAGATCATAACTCGAGTTATGATCTTCACTTTGCCGC-3'

Other clones with same target sequence:

(none)