Construct: shRNA TRCN0000361479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009289.3-4275s21c1
DNA Barcode:
TGCTATAGAATTGGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slk (20874)
Gene Description:
STE20-like kinase
Transcript:
RefSeq NM_009289.3 (CURRENT)
Match location:
Position 4275 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTATAGAATTGGGATAATT
Hairpin Sequence:
5'-CCGG-TGCTATAGAATTGGGATAATT-CTCGAG-AATTATCCCAATTCTATAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTATAGAATTGGGATAATTCTCGAGAATTATCCCAATTCTATAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTATAGAATTGGGATAATTCTCGAGAATTATCCCAATTCTATAGCA-3'

Other clones with same target sequence:

(none)