Construct: shRNA TRCN0000361494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019392.2-851s21c1
DNA Barcode:
CTTGTGAAGCCCGCAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tyro3 (22174)
Gene Description:
TYRO3 protein tyrosine kinase 3
Transcript:
RefSeq NM_019392.2 (CURRENT)
Match location:
Position 851 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTGAAGCCCGCAACATAA
Hairpin Sequence:
5'-CCGG-CTTGTGAAGCCCGCAACATAA-CTCGAG-TTATGTTGCGGGCTTCACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTGAAGCCCGCAACATAACTCGAGTTATGTTGCGGGCTTCACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTGAAGCCCGCAACATAACTCGAGTTATGTTGCGGGCTTCACAAG-3'

Other clones with same target sequence:

(none)