Transcript: Mouse NM_019392.2

Mus musculus TYRO3 protein tyrosine kinase 3 (Tyro3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Tyro3 (22174)
Length:
4005
CDS:
277..2919

Additional Resources:

NCBI RefSeq record:
NM_019392.2
NBCI Gene record:
Tyro3 (22174)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146050 GGAGTTTGACCATCCACACG pXPR_003 TGG 1705 65% 14 0.2061 Tyro3 TYRO3 76228
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019392.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361494 CTTGTGAAGCCCGCAACATAA pLKO_005 851 CDS 100% 13.200 18.480 N Tyro3 n/a
2 TRCN0000361563 AGACCTAGCAGCTCGGAATTG pLKO_005 2208 CDS 100% 10.800 15.120 N Tyro3 n/a
3 TRCN0000023528 GCGAATGGAACTGGAGAACAT pLKO.1 2592 CDS 100% 4.950 6.930 N Tyro3 n/a
4 TRCN0000023525 GCCCGATCTTTCAATCGAGAA pLKO.1 1684 CDS 100% 4.050 5.670 N Tyro3 n/a
5 TRCN0000023527 CGCCATATGCTGGCATTGAAA pLKO.1 2438 CDS 100% 0.000 0.000 N Tyro3 n/a
6 TRCN0000361564 GGCTGAGCTGCTCCTACTTTA pLKO_005 3269 3UTR 100% 13.200 9.240 N Tyro3 n/a
7 TRCN0000361566 TCATTGCCTCAAGCGACATAG pLKO_005 1913 CDS 100% 10.800 7.560 N Tyro3 n/a
8 TRCN0000023526 GCAGCTTGCATGAAGGAGTTT pLKO.1 1951 CDS 100% 4.950 3.465 N Tyro3 n/a
9 TRCN0000023524 CCTCAGAATTTCCATGCCATT pLKO.1 1222 CDS 100% 4.050 2.835 N Tyro3 n/a
10 TRCN0000231525 TCATTGCCTCAAGCGACATTG pLKO_005 1913 CDS 100% 10.800 7.560 N TYRO3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019392.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14871 pDONR223 0% 86.9% 88.6% None (many diffs) n/a
2 ccsbBroad304_14871 pLX_304 0% 86.9% 88.6% V5 (many diffs) n/a
3 TRCN0000479963 TCCTTCCTGGCGGTACGGTCCTAA pLX_317 13.4% 86.8% 88.7% V5 (many diffs) n/a
4 ccsbBroadEn_15617 pDONR223 0% 86.8% 88.6% None (many diffs) n/a
5 ccsbBroad304_15617 pLX_304 0% 86.8% 88.6% V5 (many diffs) n/a
6 TRCN0000488607 GTCTTAGTGAACTGCAAAAATAAT pLX_317 14.6% 86.8% 88.6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489876 GCATTCACCTTCAGGTGAGCCTTG pLX_317 15.5% 86.7% 88.5% V5 (many diffs) n/a
8 ccsbBroadEn_13975 pDONR223 100% 86.7% 88.3% None (many diffs) n/a
9 ccsbBroad304_13975 pLX_304 0% 86.7% 88.3% V5 (not translated due to frame shift) (many diffs) n/a
10 TRCN0000474009 TGCGGAACTACTAATCCGCATTTC pLX_317 9.6% 86.7% 88.3% V5 (not translated due to frame shift) (many diffs) n/a
11 TRCN0000487789 GGTACCGAAATCCGACTACACCGA pLX_317 21.1% 43% 44.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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