Construct: shRNA TRCN0000361512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011945.2-4480s21c1
DNA Barcode:
TCCGTACCACGTGGTAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k1 (26401)
Gene Description:
mitogen-activated protein kinase kinase kinase 1
Transcript:
RefSeq NM_011945.2 (CURRENT)
Match location:
Position 4480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGTACCACGTGGTAGTTAA
Hairpin Sequence:
5'-CCGG-TCCGTACCACGTGGTAGTTAA-CTCGAG-TTAACTACCACGTGGTACGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGTACCACGTGGTAGTTAACTCGAGTTAACTACCACGTGGTACGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGTACCACGTGGTAGTTAACTCGAGTTAACTACCACGTGGTACGGA-3'

Other clones with same target sequence:

(none)