Construct: shRNA TRCN0000361792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183294.2-842s21c1
DNA Barcode:
CCCAGGCACCAGCAAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkl1 (71091)
Gene Description:
cyclin-dependent kinase-like 1 (CDC2-related kinase)
Transcript:
RefSeq NM_183294.2 (CURRENT)
Match location:
Position 842 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGCACCAGCAAGTATTT
Hairpin Sequence:
5'-CCGG-CCCAGGCACCAGCAAGTATTT-CTCGAG-AAATACTTGCTGGTGCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGCACCAGCAAGTATTTCTCGAGAAATACTTGCTGGTGCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGCACCAGCAAGTATTTCTCGAGAAATACTTGCTGGTGCCTGGG-3'

Other clones with same target sequence:

(none)