Transcript: Mouse NM_183294.2

Mus musculus cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Cdkl1 (71091)
Length:
1689
CDS:
176..1234

Additional Resources:

NCBI RefSeq record:
NM_183294.2
NBCI Gene record:
Cdkl1 (71091)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_183294.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023211 CCACTGTAGTACCAAGAGATT pLKO.1 1189 CDS 100% 4.950 6.930 N Cdkl1 n/a
2 TRCN0000361791 GCTGTTGCAGCATCCATATTT pLKO_005 1015 CDS 100% 15.000 10.500 N Cdkl1 n/a
3 TRCN0000361792 CCCAGGCACCAGCAAGTATTT pLKO_005 842 CDS 100% 13.200 9.240 N Cdkl1 n/a
4 TRCN0000361850 CTGCCCACAGCTACAACATTA pLKO_005 1495 3UTR 100% 13.200 9.240 N Cdkl1 n/a
5 TRCN0000361790 GTGATGAAGAATTGATCAATA pLKO_005 1247 3UTR 100% 13.200 9.240 N Cdkl1 n/a
6 TRCN0000023210 CCTGTAGATGTCTGGGCAATT pLKO.1 719 CDS 100% 10.800 7.560 N Cdkl1 n/a
7 TRCN0000023209 CCAAGAGATTTAACTACCATT pLKO.1 1200 CDS 100% 4.950 3.465 N Cdkl1 n/a
8 TRCN0000023212 TGCCATAAACATAACTGCATA pLKO.1 524 CDS 100% 4.950 3.465 N Cdkl1 n/a
9 TRCN0000023213 GCCATCAAGAGGTTTCTGGAA pLKO.1 266 CDS 100% 2.640 1.848 N Cdkl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_183294.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02021 pDONR223 100% 84.4% 88.5% None (many diffs) n/a
2 ccsbBroad304_02021 pLX_304 0% 84.4% 88.5% V5 (many diffs) n/a
3 TRCN0000477527 TGTAACCGATCCCACACATCGTGG pLX_317 34.4% 84.4% 88.5% V5 (many diffs) n/a
4 TRCN0000470267 AATACCCGCGTAGATCGAACGGGA pLX_317 41.7% 73.3% 78.1% V5 (many diffs) n/a
5 ccsbBroadEn_14914 pDONR223 100% 73.2% 77.9% None (many diffs) n/a
6 ccsbBroad304_14914 pLX_304 0% 73.2% 77.9% V5 (many diffs) n/a
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