Construct: shRNA TRCN0000361919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130860.3-498s21c1
DNA Barcode:
TCTGAGATCAAGAGAGTGATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk9 (107951)
Gene Description:
cyclin-dependent kinase 9 (CDC2-related kinase)
Transcript:
RefSeq NM_130860.3 (CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGAGATCAAGAGAGTGATG
Hairpin Sequence:
5'-CCGG-TCTGAGATCAAGAGAGTGATG-CTCGAG-CATCACTCTCTTGATCTCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGAGATCAAGAGAGTGATGCTCGAGCATCACTCTCTTGATCTCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGAGATCAAGAGAGTGATGCTCGAGCATCACTCTCTTGATCTCAGA-3'

Other clones with same target sequence:

(none)