Construct: shRNA TRCN0000362253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172891.2-2142s21c1
DNA Barcode:
TCCTAGACAAATGACTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Styk1 (243659)
Gene Description:
serine/threonine/tyrosine kinase 1
Transcript:
RefSeq NM_172891.2 (CURRENT)
Match location:
Position 2142 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTAGACAAATGACTATATA
Hairpin Sequence:
5'-CCGG-TCCTAGACAAATGACTATATA-CTCGAG-TATATAGTCATTTGTCTAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTAGACAAATGACTATATACTCGAGTATATAGTCATTTGTCTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTAGACAAATGACTATATACTCGAGTATATAGTCATTTGTCTAGGA-3'

Other clones with same target sequence:

(none)