Construct: shRNA TRCN0000362330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172891.2-1859s21c1
DNA Barcode:
TCCCACCAGCATCCTACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Styk1 (243659)
Gene Description:
serine/threonine/tyrosine kinase 1
Transcript:
RefSeq NM_172891.2 (CURRENT)
Match location:
Position 1859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCACCAGCATCCTACAATA
Hairpin Sequence:
5'-CCGG-TCCCACCAGCATCCTACAATA-CTCGAG-TATTGTAGGATGCTGGTGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCACCAGCATCCTACAATACTCGAGTATTGTAGGATGCTGGTGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCACCAGCATCCTACAATACTCGAGTATTGTAGGATGCTGGTGGGA-3'

Other clones with same target sequence:

(none)