Construct: shRNA TRCN0000362420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007670.4-357s21c1
DNA Barcode:
GCCCAATCCAGGTCATGATGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkn2b (12579)
Gene Description:
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Transcript:
RefSeq NM_007670.4 (CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAATCCAGGTCATGATGA
Hairpin Sequence:
5'-CCGG-GCCCAATCCAGGTCATGATGA-CTCGAG-TCATCATGACCTGGATTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAATCCAGGTCATGATGACTCGAGTCATCATGACCTGGATTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAATCCAGGTCATGATGACTCGAGTCATCATGACCTGGATTGGGC-3'

Other clones with same target sequence:

(none)