Construct: shRNA TRCN0000362705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011613.3-1484s21c1
DNA Barcode:
ATGATAGTGTGAAGGGTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfsf11 (21943)
Gene Description:
tumor necrosis factor (ligand) superfamily, member 11
Transcript:
RefSeq NM_011613.3 (CURRENT)
Match location:
Position 1484 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGATAGTGTGAAGGGTTAAG
Hairpin Sequence:
5'-CCGG-ATGATAGTGTGAAGGGTTAAG-CTCGAG-CTTAACCCTTCACACTATCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGATAGTGTGAAGGGTTAAGCTCGAGCTTAACCCTTCACACTATCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGATAGTGTGAAGGGTTAAGCTCGAGCTTAACCCTTCACACTATCAT-3'

Other clones with same target sequence:

(none)