Construct: shRNA TRCN0000362917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028938.2-1061s21c1
DNA Barcode:
TCAAGCCTGAATGCAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrriq3 (74435)
Gene Description:
leucine-rich repeats and IQ motif containing 3
Transcript:
RefSeq NM_028938.2 (CURRENT)
Match location:
Position 1061 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGCCTGAATGCAACATAA
Hairpin Sequence:
5'-CCGG-TCAAGCCTGAATGCAACATAA-CTCGAG-TTATGTTGCATTCAGGCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGCCTGAATGCAACATAACTCGAGTTATGTTGCATTCAGGCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGCCTGAATGCAACATAACTCGAGTTATGTTGCATTCAGGCTTGA-3'

Other clones with same target sequence:

(none)