Construct: shRNA TRCN0000363488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198111.2-423s21c1
DNA Barcode:
AGGATATTTGCGAGGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akap6 (238161)
Gene Description:
A kinase (PRKA) anchor protein 6
Transcript:
RefSeq NM_198111.2 (CURRENT)
Match location:
Position 423 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATATTTGCGAGGATATTT
Hairpin Sequence:
5'-CCGG-AGGATATTTGCGAGGATATTT-CTCGAG-AAATATCCTCGCAAATATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATATTTGCGAGGATATTTCTCGAGAAATATCCTCGCAAATATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATATTTGCGAGGATATTTCTCGAGAAATATCCTCGCAAATATCCT-3'

Other clones with same target sequence:

(none)