Construct: shRNA TRCN0000363550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019411.4-1367s21c1
DNA Barcode:
GCGACATTGTTGGTCAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2ca (19052)
Gene Description:
protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Transcript:
RefSeq NM_019411.4 (CURRENT)
Match location:
Position 1367 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACATTGTTGGTCAAGAAT
Hairpin Sequence:
5'-CCGG-GCGACATTGTTGGTCAAGAAT-CTCGAG-ATTCTTGACCAACAATGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACATTGTTGGTCAAGAATCTCGAGATTCTTGACCAACAATGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACATTGTTGGTCAAGAATCTCGAGATTCTTGACCAACAATGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000081363