Construct: shRNA TRCN0000363558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053069.5-379s21c1
DNA Barcode:
GCAGAACCATACTATTTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg5 (11793)
Gene Description:
autophagy related 5
Transcript:
RefSeq NM_053069.5 (NON-CURRENT)
Match location:
Position 379 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAACCATACTATTTGCTT
Hairpin Sequence:
5'-CCGG-GCAGAACCATACTATTTGCTT-CTCGAG-AAGCAAATAGTATGGTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAACCATACTATTTGCTTCTCGAGAAGCAAATAGTATGGTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAACCATACTATTTGCTTCTCGAGAAGCAAATAGTATGGTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000150940
  2. TRCN0000099432