Construct: shRNA TRCN0000150940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004849.1-420s1c1
DNA Barcode:
GCAGAACCATACTATTTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG5 (9474)
Gene Description:
autophagy related 5
Transcript:
RefSeq NM_004849.1 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAACCATACTATTTGCTT
Hairpin Sequence:
5'-CCGG-GCAGAACCATACTATTTGCTT-CTCGAG-AAGCAAATAGTATGGTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAACCATACTATTTGCTTCTCGAGAAGCAAATAGTATGGTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAACCATACTATTTGCTTCTCGAGAAGCAAATAGTATGGTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000363558
  2. TRCN0000099432