Construct: shRNA TRCN0000363747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002117.4-841s21c1
DNA Barcode:
GCAGAGATACACGTGCCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HLA-C (3107)
Gene Description:
major histocompatibility complex, class I, C
Transcript:
RefSeq NM_002117.4 (NON-CURRENT)
Match location:
Position 841 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGATACACGTGCCATAT
Hairpin Sequence:
5'-CCGG-GCAGAGATACACGTGCCATAT-CTCGAG-ATATGGCACGTGTATCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGATACACGTGCCATATCTCGAGATATGGCACGTGTATCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGATACACGTGCCATATCTCGAGATATGGCACGTGTATCTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000057315