Construct: shRNA TRCN0000363891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018799.2-586s21c1
DNA Barcode:
AGAGGTGTTGGTGAACGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif3i (54709)
Gene Description:
eukaryotic translation initiation factor 3, subunit I
Transcript:
RefSeq NM_018799.2 (CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGTGTTGGTGAACGTAAA
Hairpin Sequence:
5'-CCGG-AGAGGTGTTGGTGAACGTAAA-CTCGAG-TTTACGTTCACCAACACCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGTGTTGGTGAACGTAAACTCGAGTTTACGTTCACCAACACCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGTGTTGGTGAACGTAAACTCGAGTTTACGTTCACCAACACCTCT-3'

Other clones with same target sequence:

(none)