Construct: shRNA TRCN0000365555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164478.1-245s21c1
DNA Barcode:
GTGTTGACCTTATTCACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C5orf63 (401207)
Gene Description:
chromosome 5 open reading frame 63
Transcript:
RefSeq NM_001164478.1 (CURRENT)
Match location:
Position 245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTGACCTTATTCACAAAG
Hairpin Sequence:
5'-CCGG-GTGTTGACCTTATTCACAAAG-CTCGAG-CTTTGTGAATAAGGTCAACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTGACCTTATTCACAAAGCTCGAGCTTTGTGAATAAGGTCAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTGACCTTATTCACAAAGCTCGAGCTTTGTGAATAAGGTCAACAC-3'

Other clones with same target sequence:

(none)