Construct: shRNA TRCN0000366056

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010153.1-1023s21c1
DNA Barcode:
GACCGTGGACTCTAGCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erbb3 (13867)
Gene Description:
erb-b2 receptor tyrosine kinase 3
Transcript:
RefSeq NM_010153.1 (CURRENT)
Match location:
Position 1023 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366056)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCGTGGACTCTAGCAATAT
Hairpin Sequence:
5'-CCGG-GACCGTGGACTCTAGCAATAT-CTCGAG-ATATTGCTAGAGTCCACGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCGTGGACTCTAGCAATATCTCGAGATATTGCTAGAGTCCACGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCGTGGACTCTAGCAATATCTCGAGATATTGCTAGAGTCCACGGTC-3'

Other clones with same target sequence:

(none)