Construct: shRNA TRCN0000366194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144862.3-188s21c1
DNA Barcode:
AGGGACTCTTCTACGAGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lims2 (225341)
Gene Description:
LIM and senescent cell antigen like domains 2
Transcript:
RefSeq NM_144862.3 (CURRENT)
Match location:
Position 188 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGACTCTTCTACGAGTTTG
Hairpin Sequence:
5'-CCGG-AGGGACTCTTCTACGAGTTTG-CTCGAG-CAAACTCGTAGAAGAGTCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGACTCTTCTACGAGTTTGCTCGAGCAAACTCGTAGAAGAGTCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGACTCTTCTACGAGTTTGCTCGAGCAAACTCGTAGAAGAGTCCCT-3'

Other clones with same target sequence:

(none)