Construct: shRNA TRCN0000366504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013779.2-2961s21c1
DNA Barcode:
TTTGCTCTGCAGGATCCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magel2 (27385)
Gene Description:
melanoma antigen, family L, 2
Transcript:
RefSeq NM_013779.2 (CURRENT)
Match location:
Position 2961 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGCTCTGCAGGATCCTTAT
Hairpin Sequence:
5'-CCGG-TTTGCTCTGCAGGATCCTTAT-CTCGAG-ATAAGGATCCTGCAGAGCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGCTCTGCAGGATCCTTATCTCGAGATAAGGATCCTGCAGAGCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGCTCTGCAGGATCCTTATCTCGAGATAAGGATCCTGCAGAGCAAA-3'

Other clones with same target sequence:

(none)