Construct: shRNA TRCN0000367006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164370.1-1161s21c1
DNA Barcode:
TACAGCTTGCACAAATCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mipol1 (73490)
Gene Description:
mirror-image polydactyly 1
Transcript:
RefSeq NM_001164370.1 (CURRENT)
Match location:
Position 1161 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACAGCTTGCACAAATCATTA
Hairpin Sequence:
5'-CCGG-TACAGCTTGCACAAATCATTA-CTCGAG-TAATGATTTGTGCAAGCTGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACAGCTTGCACAAATCATTACTCGAGTAATGATTTGTGCAAGCTGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACAGCTTGCACAAATCATTACTCGAGTAATGATTTGTGCAAGCTGTA-3'

Other clones with same target sequence:

(none)